Hearing exhibits

Proposal 231 – Verification of STRmix v2.8.0 Report, 03.2021

Attachments:

• Excel workbook
• Excel workbook
• Excel workbook

• EXH 129.1 - Signed statement –Catherine Allen
• EXH 129.2 - Signed statement –Justin Howes
• EXH 129.3 - Signed statement –Allan McNevin
• EXH 129.4 - Signed statement –Thomas Nurthen
• EXH 129.5 - Expert Report –Linzi Wilson-Wilde OAM
• EXH 129.6 - Appendix 1–Instructions to Expert and Index to Brief to Expert
• EXH 129.7 - Appendix 2–index of information provided and considered
• EXH 129.8 - Appendix 3–CV (Linzi Wilson-Wilde OAM)
• EXH 129.9 - Analytical Issues Log
• EXH 129.10 - Audit 8227 “Process audit of the automated DNA IQ System (including Off-Deck Lysis)” (Cheng, Clausen, Muharam)
• EXH 129.11 - SOP#24897v1–Automated DNA IQ Method of Extracting DNA from Blood and Cell Substrates.
• EXH 129.12 - SOP#24897v2–DNA IQ Method of Extracting DNA from Casework and Reference Samples.
• EXH 129.13 - SOP#24897v3 –DNA IQ Method of Extracting DNA from Casework and Reference Samples.
• EXH 129.14 - SOP#24897v6–DNA IQ Method of Extracting DNA from Casework and Reference Samples.
• EXH 129.15 - Report, Dr Sloots & Whiley -Review of Operations –Forensic Services, CaSS.
• EXH 129.16 - SOP #24896v1 –Automated DNA Extraction with the DNA IQ Kit Training Module.
• EXH 129.17 - SOP #24896v2 –Automated DNA Extraction with the DNA IQ Kit Training Module.
• EXH 129.18 - SOP #24896v3 –DNA Extraction with DNA IQ Kit Training Module.
• EXH 129.19 - Presentation -Audit 8227: Process Audit of the Automated DNA IQ System (including Off-Deck Lysis)
• EXH 129.20 - SOP #23602v3–Environmental Monitoring
• EXH 129.21 - SOP #22857v2 –Anti Contamination Procedure
• EXH 129.22 - Spreadsheet(FBE-07-08)
• EXH 129.23 - Extraction Batch Audit
• EXH 129.24 - Report -Audit 9642: ‘DNA IQ method of extracting DNA from casework and reference samples audit’ (Sultana & Brady)
• EXH 129.25 - Promega Technical Bulletin296,“DNA IQ™System –Small Sample Casework Protocol”
• EXH 129.26 - Promega Technical Bulletin297,“DNA IQ™System –Database Protocol
• EXH 129.27 - OQI #18580
• EXH 129.28 - OQI #19349
• EXH 129.29 - OQI #19477
• EXH 129.30 - OQI #19767
• EXH 129.31 - OQI #19768
• EXH 129.32 - OQI #20231
• EXH 129.33 - Audit #8752
• EXH 129.34 - OQI #20351
• EXH 129.35 - OQI #20367
• EXH 129.36 - OQI #20368
• EXH 129.37 - OQI #20369
• EXH 129.38 - OQI #20422
• EXH 129.39 - OQI #20432
• EXH 129.40 - OQI #20437
• EXH 129.41 - OQI #20615
• EXH 129.42 - OQI #20617
• EXH 129.43 - OQI #20690
• EXH 129.44 - OQI #20925
• EXH 129.45 - OQI #21050
• EXH 129.46 - OQI #21222
• EXH 129.47 - OQI #21309
• EXH 129.48 - Audit #9175
• EXH 129.49 - OQI #21589
• EXH 129.50 - OQI #21718
• EXH 129.51 - OQI #22438
• EXH 129.52 - Audit #9642
• EXH 129.53 - List of OQI affected cases
• EXH 129.54 - Report –‘Investigation into a partial DNA profile negative extraction control sample’ (Cheng, McNevin)
• EXH 129.55 - Report –A review of DNA extraction control results obtained in the first six months of 2008’ (Harvey & McNevin)
• EXH 129.56 - Report –A review of DNA extraction control results obtained in the second six months of 2008’ (Harvey & McNevin)
• EXH 129.57 - Report –Investigation into contamination of a negative extraction control with a positive control’
• EXH 129.58 - Report –Investigation into mixture found in FTA evidence sample
• EXH 129.59 - Report –Investiation into negative control with peaks
• EXH 129.60 - Report –Investigation into negative extraction control with a partial DNA profile
• EXH 129.61 - Report –Investigation into positive control with extra peaks
• EXH 129.62 - Report (Desley Pitcher) –DNA Extraction Modifications
• EXH 129.63 - Report (Desley Pitcher) –DNA Extraction Modifications
• EXH 129.64 - Management Team Minutes (Extraordinary Meeting)
• EXH 129.65 - Memorandum –Vanessa Ientile –DNA IQ Extractions
• EXH 129.66 - Management Team Minutes (Extraordinary Meeting)
• EXH 129.67 - Presentation –Audit 8227: Process audit of the DNA IQ System
• EXH 129.68 - Presentation –MPII Enhancements
• EXH 129.69 - Presentation – “Update on DNA Analysis Issues”
• EXH 129.70 - Correspondence from Cathie Allen to Department of Justice and Attorney-General
• EXH 129.71 - Extraction Batch Contamination – OQI #20422
• EXH 129.72 - Extraction Batch Contamination – OQI #20437
• EXH 129.73 - Extraction Batch Contamination – OQI #20615
• EXH 129.74 - Extraction Batch Contamination – OQI #20690
• EXH 129.75 - Extraction Batch Contamination – OQI #20925
• EXH 129.76 - Extraction Batch Contamination – OQI #21050
• EXH 129.77 - Extraction Batch Contamination – OQI #21222
• EXH 129.78 - Extraction Batch Contamination – OQI #21309
• EXH 129.79 - MPII ExtA Calibration 2007
• EXH 129.80 - MPII ExtA Diary 2007 Reference
• EXH 129.81 - MPII ExtA Diary 2008
• EXH 129.82 - MPII ExtA Diary 2009 (Jan-May)
• EXH 129.83 - MPII ExtA Maintenance Log 2007
• EXH 129.84 - MPII ExtA Maintenance Log 2008
• EXH 129.85 - MPII ExtA Maintenance Log 2009
• EXH 129.86 - MPII ExtB Calibration 2007
• EXH 129.87 - MPII ExtB Diary 2007 (Oct-Dec)
• EXH 129.88 - MPII ExtB Diary 2008
• EXH 129.89 - MPII ExtB Diary 2009 (Jan-May)
• EXH 129.90 - MPII ExtB Maintenance Log 2007
• EXH 129.91 - MPII ExtB Maintenance Log 2008
• EXH 129.92 - MPII ExtB Maintenance Log 2009
• EXH 129.93 - Project 9 – Report on the evaluation of commercial DNA extraction chemistries.
• EXH 129.94 - Project 11 – Report on the validation of a manual method of extracting DNA using the DNA IQ System.
• EXH 129.95 - Project 13 – Report on the Verification of an Automated DNA IQ Protocol using the Multiprobe II PLUS HT EX with Gripper Integration Platform.
• EXH 129.96 - Project 21 – A Modified DNA IQ Method Consisting of Off-Deck Lysis to Allow Supernatant Retention for Presumptive Identification of a-Amylase.
• EXH 129.97 - Project 22 – A Modified DNA IQ Method for Off-Deck Lysis Prior to performing Automated DNA Extraction

Supplementary statement of Lara Keller, 21.10.2022, including annexures ‘LK-106’ to ‘LK-145’

EXH 131a - Employee movement form

Statement of Justin Howes, 09.08.2022.

Including annexures JH-1 to JH-36.

Statement of Justin Howes, 16.09.2022.

Including annexures ‘JH-1’ to ‘JH-11’.

Statement of Catherine Allen, 16.09.2022, including:

• annexures 'CA-01’ to ‘CA-152’: Part 1
• annexures 'CA-01’ to ‘CA-152’: Part 2

Statement of Catherine Allen, 19.09.2022.

Including annexures 'CA-01’ to ‘CA-91’.

Statement of Alicia Quartermain dated 21.09.2022.

Including Annexures ‘AQ-01’ to ‘AQ-07’.

Statement of Alicia Quartermain dated 6.10.2022.

Including Annexure ‘AQ-01’.

Statement of Angelina Keller, dated 6.10.2022, including annexures ‘AK-01’ to ‘AK-41’

• EXH 89.1 - Project Proposal #152 - Validation of Quantifiler Trio February 2015
• EXH 89.2 - Validation of Quantifiler® Trio
• EXH 89.3 - Project Proposal #185 - Validation of two QuantStudio 5 Real-Time PCR Systems
• EXH 89.4 - Project Report #185 - Validation of two QuantStudio 5 Real-Time PCR Systems
• EXH 89.5 - Validation of Quant Studio 5
• EXH 89.6 - 3500xL Genetic Analyzer Validation for Reference samples Amplified with Powerplex®21 using Direct Amplification
• EXH 89.7 - Genetic Analyzer Validation for Extracted Reference Samples Amplified with PowerPlex®21
• EXH 89.8 - 3500xL Genetic Analyzer Validation for Casework Samples Amplified with PowerPlex®21
• EXH 89.9 - Project Final Report #145 - Verification of 3500xL B
• EXH 89.10 - Project #182 - Validation of 3500xL Analysis of Casework PowerPlex®21 WEN
• EXH 89.11 - Project Report #186 - Assessment of 3500xl A Genetic Analyzer for Processing Casework PowerPlex® Samples, Version 2.0
• EXH 89.12 - Project Proposal #173 - Validation of Hamilton STARlet A for Quantification and amplification Assay Setup
• EXH 89.13 - Project Proposal #173 - Verification of Hamilton STARlet B for Quantification and Amplification Assay Setup
• EXH 89.14 - Project Final Report #175 Validation of Hamilton® STARlet C for Capillary Electrophoresis Setup
• EXH 89.15 - Project Report #199 - Verification of ProFlex^TM96 Well PCR System using PowerPlex®21 December 2021 Version 2.0
• EXH 89.16 - Summary Model Maker results for Project #199
• EXH 89.17 - Minor Process Change - Summary Model Maker results for Project #199
• EXH 89.18 - Validation of the QIAsymphony® SP/AS Modules
• EXH 89.19 - Project Report #192 - Validation of QIAsymphony SP for bone extraction
• EXH 89.20 - Project Report #192 - Validation of QIAsymphony SP for Bone Extraction - Supplementary Repeatability and Reproducibility
• EXH 89.21 - Project #148 to optimise the cleaning protocol for bone crusher vials
• EXH 89.22 - 23402V10 Writing Guidelines for Validation and Change Management Reports
• EXH 89.23 - 23401V8 Forensic DNA Analysis Validation and Verification Guidelines
• EXH 89.24 - 17117V21 Procedure for Case Management
• EXH 89.25 - 33407V3 Quantification of Extracted DNA using the Quantifiler® Trio DNA Quantification Kit
• EXH 89.26 - 34045V7 Quantification of Extracted DNA using the Quantifiler® Trio DNA Quantification Kit (later version)
• EXH 89.27 - Email from Cathie Allen to Kirsten ScottRE: Mixture (male: Female) results from Quant Trio - feedback by 5pm 15th May.
• EXH 89.28 - Email from Kaye Ballantyne to Justin Howes, 'RE Validation question'
• EXH 89.29 - Email from Sharon Johnstone to Kylie Rika, 'RE quant trio'
• EXH 89.30 - Email from Megan Mathieson to Kylie Rika, 'RE quant trio and dilutions'
• EXH 89.31 - Email from Cathie Allen to Luke Ryan, 'RE: Quantifiler Trio Issue'
• EXH 89.32 - Email from Kylie Rika to Rhys Parry, 'RE: follow up'
• EXH 89.33 - Email from Justin Howes to Emma Caunt, 'RE: Model Maker for Proflexes'
• EXH 89.34 - Professor Linzi Wilson-Wilde OAM PhD, Opinion as to the accuracy of statements made by reporting scientist in formal witness statements regarding samples that have "no DNA detected".
• EXH 89.35 - Bruce Budowle PhD, Review and Assessment of the Use of "NO DNA Detected" by Queensland Health Forensic and Scientific Services (QHFSS).

• EXH 91.1 - Signed statement – Allan McNevin
• EXH 91.2 - Signed statement – Allan McNevin
• EXH 91.3 - Signed statement – Allan McNevin
• EXH 91.4 - Signed statement – Jacqueline Wilson
• EXH 91.5 - Signed statement – Matthew Hunt
• EXH 91.6 - Signed statement – Chelsea Savage
• EXH 91.7 - Signed statement – Catherine Allen
• EXH 91.8 - Signed statement – Justin Howes
• EXH 91.9 - Expert Report – Clint Cochrane
• EXH 91.10 - Appendix A – CV (Clint Cochrane)
• EXH 91.11 - Appendix B – Instructions to Expert and Index to Brief to Expert
• EXH 91.12 - Initial Project Request Form
• EXH 91.13 - Email from Allan McNevin
• EXH 91.14 - Project Proposal #181 – Signed
• EXH 91.15 - Project Proposal #181 (Part 2) – Signed
• EXH 91.16 - Project Proposal #181 (Part 3) – Signed
• EXH 91.17 - Project Proposal #181 (Part 4) – Signed
• EXH 91.18 - Project Proposal #181 (Part 4 – Additional Testing) – Signed
• EXH 91.19 - Project #181 – Spermatozoa Microscopy Sensitivity – Final Report – Signed
• EXH 91.20 - Report – Data Analysis of modified sexual assault process for zero spermatozoa detected at Evidence Recovery (Brisotto, Hunt, Rika, Ryan)
• EXH 91.21 - ESR - Scientific Review/Terms of Reference
• EXH 91.22 - ESR Report
• EXH 91.23 - SOP 17189v10 – Examination for and of Spermatozoa
• EXH 91.24 - SOP 17189v13 – Examination for and of Spermatozoa
• EXH 91.25 - Email from Jacqueline Wilson to Amanda Reeves and Justin Howes
• EXH 91.26 - Email from Justin Howes to Jacqueline Wilson and Amanda Reeves
• EXH 91.27 - Email from Justin Howes to Jacqueline Wilson and Amanda Reeves
• EXH 91.28 - Email from Allan McNevin to Amanda Reeves
• EXH 91.29 - Email from Kylie Rika to others
• EXH 91.30 - Email from Amanda Reeves to Justin Howes
• EXH 91.31 - Email from Allan McNevin to Paula Brisotto
• EXH 91.32 - Project Plan (Project #181)
• EXH 91.33 - Email from Adrian Pippia to Justin Howes, Amanda Reeves and Kylie Rika
• EXH 91.34 - Email Kylie Rika to Amanda Reeves and Justin Howes
• EXH 91.34a - Email from Justin Howes to Allan McNevin
• EXH 91.35 - Email from Allan McNevin to others (Evidence Recovery Team)
• EXH 91.36 - Project Proposal - Project #181
• EXH 91.37 - Brief for Approval – Engagement of Livingstones – Signed by Gary Uhlmann
• EXH 91.38 - Emails between Cathie Allen and Paul Csoban
• EXH 91.39 - Contract for Services - ESR
• EXH 91.40 - Letter from Paul Csoban to Amanda Reeves
• EXH 91.41 - Emails between Catherine Allen and Paul Csoban
• EXH 91.42 - Director-General Brief for Noting
• EXH 91.43 - Letter from Gary Uhlmann to Amanda Reeves
• EXH 91.44 - Livingstones Report – External Investigation into DNA Analysis Team - QHFSS
• EXH 91.45 - Project #181 – Draft Interim Report
• EXH 91.46 - Email from Douglas Elliot to Cathie Allen
• EXH 91.47 - Email from Paul Csoban to Jade Franklin, Karyn Bell, Sharon Kelly and Cathie Allen
• EXH 91.48 - Clayton Utz advice – Amanda Reeves management of employment issues
• EXH 91.49 - Email from Jade Franklin to Paul Csoban
• EXH 91.50 - Email from Cathie Allen to Paul Csoban and Jade Franklin
• EXH 91.51 - Email from Paul Csoban to Gary Uhlmann and Jade Franklin
• EXH 91.52 - Email from Gary Uhlmann to Paul Csoban and Cathie Allen

Statement of Kylie Dale Rika dated 09.08.2022.

Including annexures KDR-1 to KDR-6.

Statement of Kylie Dale Rika dated 16.09.20.

Including annexures KR-01 to KR-19.

Statement of David Harold Neville dated 26.08.2022

Forensic DNA Analysis Team Chart as at 27.06.2022

Project 184 project plan

Email from Justin Howes to reviewing scientists, ‘Project #184 for review’, 30.11.2017.

Project 184 report

Excel spreadsheet: Options Paper Feedback - Feedback on versions, December 2017.

Email from Dale Frieberg to Ewen Taylor, ‘FW: Options Paper for consideration’, 30.01.2018.

A review of the automatic concentration of DNA extracts: using Microcon Centrifugal Filter Devices: Options for QPS consideration, January 2018.

Email form Lara Keller to Shaun Drummond, ‘Forensic DNA testing impacts’, 03.06.2022.

Statement of David Harold Neville dated 14.09.2022

Email from Helen Gregg to David Rosengren, ‘Wording to describe pre-2018 thresholds and options’, 17.08.2022.

Proposal #163: Assessment of results obtained from ‘auto-microcon’ samples - Project Plan (signed), June 2015.

Email from Justin Howes to reporting scientists, ‘Assistance in TAT reduction’, 27.03.2015.

Initial Request, Proposal #163, ‘Assessment of results obtained from auto-microcon samples’, 01.04.2015.

Assessment of results obtained from ‘automatic microcon’ samples, August 2015.

Email from Justin Howes to Thomas Nurthen, Kylie Rika, Sharon Johnstone, Amanda Reeves, ‘RE: wording for DNA insuff’, 07.02.2018.

Email from Kylie Rika to Justin Howes, Thomas Nurthen, Sharon Johnstone, ‘RE: wording for DNA insuff’, 07.02.2018.

Email from Justin Howes to Emma Caunt, Kylie Rika, Matthew Hunt, Rhys Parry, ‘Re: Fwd: amp differences’, 02.10.2013.

Addendum Statement of David Harold Neville dated 8 September 2022

Standard Operating Procedure 17117V19, ‘Procedure for Case Management’, valid from 24.04.2017.

Email from Kylie Rika to Justin Howes, ‘RE: 1092496630’, 03.06.2020

Report into the QHFSS Options Paper

Australian Standard ISO/IEC 17025:2018, ‘General requirements for the competence of testing and calibration laboratories’.

The Evaluation of the Efficacy of a Post-Extraction Concentration Step Using the Microcon Centrifugal Filter Devices in Yielding DNA Profile Intelligence, January 2018.

A review of the automatic concentration of DNA extracts: using Microcon Centrifugal Filter Devices: Options for QPS consideration, January 2018.

Review and Assessment of the Appropriateness of Not Concentrating Low Quantity DNA Samples by Queensland Health Forensic and Scientific Services (QHFSS), Bruce Budowle, Ph. D., 15.09.2022.

Assessment of the Options Paper and Update Paper Prepared by Queensland Health Forensic and Scientific Services (QHFSS), Bruce Budowle, Ph. D., 19.09.2022.

Email chain between Catherine Allen and Dale Frieberg ‘RE: DNA Profiling Kits’, 06.12.2017

Options Paper: ‘Considerations for a new DNA profiling kit for use on casework  samples in Forensic DNA Analysis’, 25.10.2016

Statement of Catherine Allen dated 16.09.2022, Annexure ‘CA-103’:
Email chain between Catherine Allen and Dale Frieberg, ‘RE: Volume Crime Samples’, 22.01.2018

Statement of Catherine Allen dated 16.09.2022, Annexure ‘CA-104’:
Email chain between Catherine Allen and Dale Frieberg, ‘RE: Options Paper for Consideration’, 30.01.2018

Statement of Ewen Taylor, dated 23rd August 2022, including annexures ‘Exhibit 1’ to ‘Exhibit 7’

Statement of Paul Csoban dated 15th September 2022, including annexures ‘PC-1 to ‘PC-2’

Statement of Detective Sergeant [withheld] dated 20.09.2022, including annexures 1 to 6

Statement of Senior Constable [withheld] dated 20.09.2022, including annexures 1 to 11

Addendum statement of Senior Constable [withheld] dated 26.09.2022, including annexure 1

Statement of Michael Lok dated 16.09.2022

Statement of Michael Walsh dated 23.09.2022, including annexures “MW-01” to “MW-03”

Statement of Stephan Paul Foxover dated 16.09.2022, including annexures ‘Exhibit 1’ to ‘Exhibit 5’

Further Statement of Darren John Pobar dated 15.09.2022, including annexures ‘Exhibit 9’ to ‘Exhibit 13’

Statement of Paula Brisotto dated 21.09.2022, including annexures ‘PB-00’ to ‘PB-128’

Initial request - restart of project #163, dated 27.04.2017

Project Proposal #184, dated 31.08.2017